Mason replaces Jose Mourinho who was relieved of his duties on Monday after an underwhelming 2020/2021 season so far.
Mason took training on Monday after news of Mourinho’s dismissal was confirmed and he will be in the dugout when Tottenham face Southampton in the Premier League on Wednesday.
The 29-year-old made 70 appearances for Tottenham’s first team, having come through their youth system but was forced to retire in 2017 while playing for Hull City after a suffering a fractured skull in a league game.
Tottenham have confirmed the news in an official statement, which reads: "Following the departure of Jose Mourinho, we can now confirm that Ryan Mason will take charge as interim head coach for the remainder of the season.
"Ryan will be joined by Chris Powell and Nigel Gibbs as interim assistant head coaches and Michel Vorm as interim goalkeeping coach. Ledley King will continue in his role as first-team assistant."
Club chairman Daniel Levy added: "We have great belief in this squad of talented players.
"We have a cup final and six Premier League games ahead of us and we shall now focus all our energies on achieving a strong finish to the season."
Mason will look to win Tottenham’s first trophy in more than a decade in Sunday’s Carabao Cup final against Manchester City.