The 'sign on' chant makes reference to unemployment and poverty issues that blighted the city of Liverpool in the 1980's.
Saturday's encounter at Anfield ended 1-1, with Spurs putting a dent on Liverpool's title aspirations.
In a statement released on the organisation’s website, THST said: “Singing about the opposition has long been a feature of English football, and we are reluctant to tell fans how they should support the team. Nevertheless, we were disappointed to hear the ‘sign on’ chant at Saturday's away match at Anfield.
“Poverty and joblessness are not fair game for banter. There is a cost of living crisis throughout England, including in London. Our club represents an area and community with the fastest growing rate of unemployment in the country.
“We are rightly proud of our away support, and we want it to be noticed for positive reasons. For most of the game at Anfield our fans gave superb backing to a great team performance. Spurs fans have provided financial backing to Marine FC on Merseyside, and regularly contribute to foodbanks and community initiatives in London. This is what we are about.
“The ‘sign on’ chant is not what we are about. We’re Tottenham Hotspur. We’re better than that.”