The Blues return to action for the first time since a 3-1 victory over Southampton in the Premier League on October 2.
Chelsea will be without several players when they make a trip to face the Bees at the Brentford Community Stadium.
Thiago Silva will not be available for the Blues as he was part of the Brazil squad that beat Uruguay 4-1 on Friday morning.
Antonio Rudiger returned from the international break with an injury, and Christian Pulisic has not recovered yet.
Morocco superstar Hakim Ziyech is also a major doubt, having missed training due to headache.
Romelu Lukaku was also a doubt when he returned early after being feared injured with Belgium but Tuchel has revealed the striker is fit to face Brentford.
"Thiago [Silva] will be back in the afternoon, hopefully safe from this match in Brazil," said Tuchel in his press conference on Friday.
"We had our talk and tactical training, he missed all that. He played 90 minutes and had 12 hours of travelling so that gives you the answer.
"Toni Rudiger is injured, as he came back injured from the national team. Christian Pulisic is still injured. We also have doubts with Hakim Ziyech who has missed the last couple of sessions with a headache.
"Romelu is fine and will be involved."
