The Blues lost at home in Frank Lampard’s managerial bow in Europe’s Premier club competition as Rodrigo Moreno’s second half goal ensured the Spanish side took all three points to Spain.
The hosts were handed a reprieve three minutes to full time when referee Cuneyt Cakir whistled for a penalty after Fikayo Tomori’s header struck the arm of Daniel Wass, but Barkley, on as a substitute six minutes earlier, sent his kick against the crossbar and out of play.
There was some deliberation between Barkley, Willian and Jorginho as to who would effect the kick before the Englishman missed but Lampard reaffirmed after the game that the former Everton man is the team’s chosen one when it comes to spot kick duties.
"Ross is the penalty taker. He was in pre-season and he scored a couple and he was when he came on," Lampard told a news conference.
"He has been when he started, was when he came on today and he missed it. That is the clear story.
"I don't know what the conversation was [between them]. Jorginho and Willian were the takers on the pitch but once Ross comes on, it's his and he missed it."
Lampard has now failed to win any of his three home games so far this season, drawing with Leicester City and Sheffield United before Tuesday night’s loss.