James accosted referee Jarred Gillett in the tunnel following Chelsea's 1-0 home defeat to Aston Villa in the Premier League last month.
The 23-year-old did not feature in the game due to a hamstring injury but made his presence felt in the wrong way after his side failed to take anything from the game.
The FA said on their Spokesperson Twitter account: "Reece James has been given a one-match suspension and £90,000 fine for breaching FA Rule E3 at Chelsea's Premier League match against Aston Villa on Sunday, September 24.
"The defender admitted that he used improper, insulting and/or abusive words and behaviour towards a match official in the tunnel after the final whistle.
"An independent Regulatory Commission imposed these sanctions following a hearing and its written reasons for them will be published at the earliest opportunity."
James will serve his ban when Chelsea travel to Burnley this weekend.
James, who was named Chelsea captain this summer, has not played since the opening Premier League game of the season against Liverpool.
