The 34-year-old has been a cornerstone of the Barcelona midfield for the best part of 15 years, having graduated from the club's famed La Masia academy in 2008.
Busquets has won countless trophies during his time at the Camp Nou, among them three UEFA Champions League trophies, eight La Liga titles which is likely to increase to nine at the end of this season, seven Copa del Reys and three UEFA Super Cups.
The former Spain international has decided not to renew his contract which expires at the end of this season.
Busquets has been linked with a move to America's Major League Soccer to continue his career.
"It has been an unforgettable journey," he said in the video announcing his departure. "Since I was a child coming to the matches, or watching them on TV, I always dreamed of playing with this shirt at this stadium.
"The reality has exceeded all my dreams. I wouldn't have believed you if you told me when I arrived as a youth player that I would play 15 seasons for the best club in the world, the club of my life, of which I have been, am and always will be a fan, member, player, captain, and surpass 700 matches."
Busquets has made 718 appearances in total for Barcelona, scoring 18 goals and providing 40 assists.
He won the 2010 FIFA World Cup and 2012 European Championship with Spain, earning 143 caps for his country.
