The Andalusian side turned to Sampaoli after sacking Julen Lopetegui back in October.
The Argentinian was previously in charge of Sevilla for the 2016/2017 season, leading them to a fourth-place finish.
But his second coming has failed inspire the required turnaround anticipated despite Sevilla reaching the quarterfinal stage of the Europa League, where they will face Manchester United.
A 2-0 defeat to Getafe over the weekend saw Sevilla drop to 14th position on the league table, just two points above the relegation zone.
“Sevilla FC have today relieved Jorge Sampaoli of his duties,” a statement on their club website read.
“Following the defeat at Getafe, which leaves the side just above the relegation zone, the club have decided to part ways with the Argentine coach ahead of the international break.”
“Sampaoli will no longer lead today’s training session, scheduled for 18.00, while the club is already working on recruiting a new coach as soon as possible.
“Sevilla FC would like to thank Jorge Sampaoli for his dedication and commitment at the club and wish him the best of luck in his future.”
Sevilla will face Cadiz right after the international break.
