The World football's governing body is currently studying a proposal which will see both men's and women's World Cup played ever two years.
And with the WLF, the association of professional football leagues, not in favor of the idea, has informed Uefa to stand against the approach.
The association is of the view that the proposal would "dilute the historical and traditional values" of the World Cup.
"A biennial World Cup would negatively disrupt the football economy and undermine players' welfare in a calendar that is already overloaded," read the World Leagues Forum's statement.
"As the employers of players and developers of the game at domestic level, leagues request full and transparent discussions so that the football calendar - which requires a complementary balance between club matches and national teams - can be agreed upon by all parties involved to benefit the game at all levels over the long term.
"Working together with all football stakeholders, the World Leagues Forum will ensure Fifa is not allowed to make unilateral decisions on the future of football against the interests of leagues, clubs, players and fans."
The idea of introducing a biennial men's and women's tournament is been led by former Arsenal boss Arsene Wenger, who is now chief of global football development at Fifa.
After the first World Cup held in 1930 in Uruguay, the men's World Cup has been held every four years except in 1942 and 1946 because of World War Two.
The women's tournament has also been every four years since it began in 1991.
