The 36-year-old Portuguese international had had problem training recently and will sit out the clash against Gian Piero Gasperini’s men as Juventus attempt to cement their place in the top four, with the league title looking out of reach.
Juventus manager Andrea Pirlo made known Ronaldo’s absence in his press conference for the clash at the Gewiss Stadium.
"Cristiano Ronaldo has a problem with his thigh flexors, he won't be there against Atalanta.
"We will try to recover him for Wednesday [against Parma].
"He wasn’t able to ‘push’ the way he wanted to in training this week and we prefer not to risk it. The internationals didn't help, he's been playing a lot lately.
"Paulo Dybala will start tomorrow."
Pirlo added: "Arthur and McKennie are fine, they are fully available for tomorrow.
"Bonucci, after Covid, is doing a specific job to get back to top shape.
"[Juan] Cuadrado can be a solution, both high and low. We have thought of various situations.
"We need to give confidence to young people, it is not a gamble and we are doing it, for example with [Dejan] Kulusevski."
Ronaldo is the Serie A’s top scorer this season with 25 goals in 27 games.