Gasperini believes their game against Los Blancos at the Gewiss Stadium was ruined when referee Tobias Stieler sent off Remo Freuler for his foul on Ferland Mendy after just 17 minutes into the game.
Mendy was put through with a sumptuous pass and he seemed to be going wide to cross the ball before the Switzerland international brought him down.
The German referee adjudged that Freuler made a professional foul when Mendy was through on goal.
Real Madrid had the numerical advantage over the Italians but were kept at bay for the vast majority of the game until Mendy curled home the winner during the dying embers.
Speaking to the media after the game, Gasperini launched a scathing attack on the decision.
“The game was ruined. Last season, after absolute chaos, the handball rule was sorted out. Now we have the temptation to remove any form of contact in football and that is suicide for the sport,” Gasperini told Sky Sport Italia.
“I just got banned for saying something in Serie A, if I say something now then UEFA will ban me for a month.
“But this is football suicide. We cannot have referees who never played the game and don’t know the difference between a challenge and a foul.
“If they can’t tell the difference, then they ought to go and get a different job, frankly. They should get people who played football to referee instead.
“Referees also have video replays now, they have everything needed to judge, and yet even with replays they get it wrong.
“You wait so long for this event and then it gets ruined. We might’ve lost anyway, I am not complaining about the result, but we at least could’ve played our game."
